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| Section | Objectives |
|---|---|
| Topic 1: Urinalysis and Body Fluids | - Fluid analysis and interpretation
|
| Topic 2: Microbiology | - Clinical microbiological techniques
|
| Topic 3: Clinical Chemistry | - Metabolic and analytical testing
|
| Topic 4: Blood Banking / Immunohematology | - Blood group systems and antibody identification
|
| Topic 5: Laboratory Operations | - Quality and safety in the clinical lab
|
| Topic 6: Hematology | - Blood cell analysis and disorders
|
1. Creatinine clearance tests are utilized to estimate the glomerular function of the kidney. The creatitine clearance calculation is defined as the volume of plasma that is cleared of creatinine by the kidney per unit of time and uses the following formula:
creatinine clearance = Urine creatinine conc x Volume / Plamsa creatinine conc Creatinine clearance measurements have a recommended specimen requirement = 24-hour urine collection.
Which of the following tests would be useful in the assessment of glomerular filtration:
A) Creatinine clearance
B) Urea clearance
C) 24 hour urine protein
D) PSP test
2. Escherichia coli and Klebsiella pneumoniae are two good quality control choices for the indole test. E. coli is indole positive, while K. pneumoniae is indole negative. A positive reaction is noted when there is a red layer at the top of the tube after the addition of Kovács reagent, while the negative result is a lack of color change in the top of the tube after the addition of Kovács reagent.
Which one of the following combinations of organisms would be appropriate positive and negative controls to verify the specific test functions listed?
A) Indole - Escherichia coli and Klebsiella pneumonia
B) Beta hemolysis - Escherichia coli and Streptococcus pyogenes
C) Carbohydrate fermentation - Alcaligenes faecalis and Acinetobacter baumannii
D) Hydrogen sulfide production - proteus mirabilis and Salmonella typhi
E) Catalase - Staphylococcus aureus and Staphylococcus epidermidis
3. A zone of inhibition is the area around an antibiotic-infused paper disk that does not show any bacterial growth. The antibiotic impregnated on the disk will diffuse into the agar in the area surrounding the disk. If the bacteria are sensitive to the antibiotic, they cannot grow near the disk. The size of the zone is proportional to how sensitive the organism is. If the organism is resistant to the antibiotic, it will grow very closely to the disk.
The size of the zone of suppressed growth on a sensitivity plate using sensitivity disks is referred to as the zone of:
A) gamma hemolysis
B) inhibition
C) alpha hemolysis
D) susceptibility
E) beta hemolysis
4. To prevent blood from clotting the specimen must be:
A) centrifuged right after collection.
B) both A and B
C) inverted right after collection.
D) collected in a tube containing an anticoagulant.
5. Although cysteine-blood agar was traditionally used, F. tularensis will also grow on commercially available Thayer-Martin and chocolate agar which have been enriched with supplemental nutrients.
Which of the following media would you use to isolate Francisella tularensis:
A) Cysteine-blood agar
B) Sheep-blood agar
C) Lowenstein-Jensen media
D) Bordet-Gengou media
Solutions:
| Question # 1 Answer: D | Question # 2 Answer: A | Question # 3 Answer: B | Question # 4 Answer: B | Question # 5 Answer: A |
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